Western blotting analysis using L1 cell adhesion molecule antibody (Cat#61991). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with L1 cell adhesion molecule antibody (Cat#61991, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using L1 cell adhesion molecule antibody (Cat#61991). L1 cell adhesion molecule expression in wild-type (WT) and L1 cell adhesion molecule (L1CAM) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with L1 cell adhesion molecule antibody (Cat#61991, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of L1 cell adhesion molecule knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with L1 cell adhesion molecule antibody (Cat#61991, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
