Western blotting analysis using Exo/Endonuclease G antibody (Cat#62853). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Exo/Endonuclease G antibody (Cat#62853, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Exo/endonuclease G antibody (Cat#62853). Exo/Endonuclease G expression in wild type (WT) and exo/endonuclease G shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Exo/endonuclease G antibody (Cat#62853, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Exo/Endonuclease G antibody (Cat#62853, 1:1,000). Nuclei were stained blue with DAPI; Exo/Endonuclease G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of exo/endonuclease G expression in HepG2 cells using exo/endonuclease G antibody (Cat#62853, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, exo/endonuclease G.
Validation of Exo/Endonuclease G knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Exo/Endonuclease G antibody (Cat#62853, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
