Western blotting analysis using phosphomevalonate kinase antibody (Cat#65856). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phosphomevalonate kinase antibody (Cat#65856, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using phosphomevalonate kinase antibody (Cat#65856). Phosphomevalonate kinase expression in wild-type (WT) and phosphomevalonate kinase (PMVK) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phosphomevalonate kinase antibody (Cat#65856, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phosphomevalonate kinase expression in C2C12 cells using Phosphomevalonate kinase antibody (Cat#65856, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phosphomevalonate kinase.
