Western blotting analysis using transaldolase 1 antibody (Cat#66377). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with transaldolase 1 antibody (Cat#66377, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using transaldolase 1 antibody (Cat#66377). Transaldolase 1 expression in wild-type (WT) and transaldolase 1 (TALDO1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with transaldolase 1 antibody (Cat#66377, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of transaldolase 1 expression in HeLa cells using transaldolase 1 antibody (Cat#66377, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Transaldolase 1.
