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KD-Validated ASH2L Recombinant Rabbit mAb

#67045

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using ASH2L antibody (Cat#67045). Total lysates (30 μg) from various cell lines and mouse testis tissue were loaded and separated by SDS-PAGE. The blot was incubated with ASH2L antibody (Cat#67045, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ASH2L antibody (Cat#67045). Total lysates (30 μg) from various cell lines and mouse testis tissue were loaded and separated by SDS-PAGE. The blot was incubated with ASH2L antibody (Cat#67045, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ASH2L antibody (Cat#67045). ASH2L expression in wild-type (WT) and ASH2L shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with ASH2L antibody (Cat#67045, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    ASH2L
  • Catalog:
    67045
  • Application:
    WB
  • Species Reactivity:
    H, R
Information
Product Name KD-Validated ASH2L Recombinant Rabbit mAb
Aliases ASH2L; ASH2 Like, Histone Lysine Methyltransferase Complex Subunit; ASH2L2; ASH2L1; ASH2; Bre2; Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2; ASH2-Like Protein; ASH2 Like Histone Lysine Methyltransferase Complex Subunit; Ash2 (Absent, Small, Or Homeotic)-Like (Drosophila); Ash2 (Absent, Small, Or Homeotic)-Like
Background

Gene Name: ASH2L

NCBI Gene Entry: 9070

UniProt Entry: Q9UBL3

Application Information

Molecular Weight: Predicted, 69 kDa; observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB2645

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB)

Conjugation: Unconjugated

Immunogen A synthesized peptide derived from human ASH2L
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using ASH2L antibody (Cat#67045). Total lysates (30 μg) from various cell lines and mouse testis tissue were loaded and separated by SDS-PAGE. The blot was incubated with ASH2L antibody (Cat#67045, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using ASH2L antibody (Cat#67045). Total lysates (30 μg) from various cell lines and mouse testis tissue were loaded and separated by SDS-PAGE. The blot was incubated with ASH2L antibody (Cat#67045, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using ASH2L antibody (Cat#67045). ASH2L expression in wild-type (WT) and ASH2L shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with ASH2L antibody (Cat#67045, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ASH2L antibody (Cat#67045). Total lysates (30 μg) from various cell lines and mouse testis tissue were loaded and separated by SDS-PAGE. The blot was incubated with ASH2L antibody (Cat#67045, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ASH2L antibody (Cat#67045). Total lysates (30 μg) from various cell lines and mouse testis tissue were loaded and separated by SDS-PAGE. The blot was incubated with ASH2L antibody (Cat#67045, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ASH2L antibody (Cat#67045). ASH2L expression in wild-type (WT) and ASH2L shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with ASH2L antibody (Cat#67045, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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