Western blotting analysis using ASH2L antibody (Cat#67045). Total lysates (30 μg) from various cell lines and mouse testis tissue were loaded and separated by SDS-PAGE. The blot was incubated with ASH2L antibody (Cat#67045, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ASH2L antibody (Cat#67045). Total lysates (30 μg) from various cell lines and mouse testis tissue were loaded and separated by SDS-PAGE. The blot was incubated with ASH2L antibody (Cat#67045, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ASH2L antibody (Cat#67045). ASH2L expression in wild-type (WT) and ASH2L shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with ASH2L antibody (Cat#67045, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).