Western blotting analysis using IGF2BP3 antibody (Cat#6883). Total lysates (30 μg) from various cell lines and mouse spleen, mouse branin tissues were loaded and separated by SDS-PAGE. The blot was incubated with IGF2BP3 antibody (Cat#6883, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with IGF2BP3 antibody (Cat#6883, 1:1,000). Nuclei were stained blue with DAPI; IGF2BP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
IGF2BP3; Insulin Like Growth Factor 2 MRNA Binding Protein 3; IMP-3; IMP3; CT98; Insulin-Like Growth Factor 2 MRNA-Binding Protein 3; IGF-II MRNA-Binding Protein 3; IGF2 MRNA-Binding Protein 3; Cancer/Testis Antigen 98; VICKZ Family Member 3; VICKZ3; KOC1; KH Domain Containing Protein Overexpressed In Cancer; KH Domain-Containing Protein Overexpressed In Cancer; Insulin-Like Growth Factor 2 MRNA Binding Protein 3; IGF II MRNA Binding Protein 3; HKOC; KOC
Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen
A synthesized peptide derived from human IGF2BP3
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:2,000-1:10,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using IGF2BP3 antibody (Cat#6883). Total lysates (30 μg) from various cell lines and mouse spleen, mouse branin tissues were loaded and separated by SDS-PAGE. The blot was incubated with IGF2BP3 antibody (Cat#6883, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with IGF2BP3 antibody (Cat#6883, 1:1,000). Nuclei were stained blue with DAPI; IGF2BP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using IGF2BP3 antibody (Cat#6883). Total lysates (30 μg) from various cell lines and mouse spleen, mouse branin tissues were loaded and separated by SDS-PAGE. The blot was incubated with IGF2BP3 antibody (Cat#6883, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with IGF2BP3 antibody (Cat#6883, 1:1,000). Nuclei were stained blue with DAPI; IGF2BP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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