Western blotting analysis using MEX3D antibody (Cat#6258). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MEX3D antibody (Cat#6258, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with MEX3D antibody (Cat#6258, 1:1,000). Nuclei were stained blue with DAPI; MEX3D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MEX3D expression in HepG2 cells using MEX3D antibody (Cat#6258,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MEX3D.
MEX3D; Mex-3 RNA Binding Family Member D; RNF193; OK/SW-Cl.4; KIAA2031; RKHD1; Tino; RING Finger And KH Domain-Containing Protein 1; Ring Finger And KH Domain Containing 1; Bcl-2 ARE RNA Binding Protein; RNA-Binding Protein MEX3D; RING Finger Protein 193; Ring Finger (C3HC4 Type) And KH Domain Containing 1; Mex-3 Homolog D (C. Elegans); MEX-3D; MEX3; TINO
Western blotting analysis using MEX3D antibody (Cat#6258). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MEX3D antibody (Cat#6258, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with MEX3D antibody (Cat#6258, 1:1,000). Nuclei were stained blue with DAPI; MEX3D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MEX3D expression in HepG2 cells using MEX3D antibody (Cat#6258,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MEX3D.
Western blotting analysis using MEX3D antibody (Cat#6258). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MEX3D antibody (Cat#6258, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with MEX3D antibody (Cat#6258, 1:1,000). Nuclei were stained blue with DAPI; MEX3D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MEX3D expression in HepG2 cells using MEX3D antibody (Cat#6258,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MEX3D.
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